Moore has been sidelined since injuring a hamstring in the Falcons' home win over New Orleans on Nov. 29. He intercepted quarterback Drew Brees twice and finished with 11 tackles.
Samuel played just three snaps before aggravating a right shoulder injury against the Saints.
In his first season with Atlanta, Samuel, a four-time Pro Bowl selection, has a team-high 13 passes defended.
The Falcons (11-1) have won five straight against Carolina (3-9).