Malkin who was placed on injured reserve Sunday, retroactive to March 10, with an upper body injury, is expected to be back in the lineup soon.
However, defenceman Kris Letang has suffered a broken toe, and was placed on the injured reserve Wednesday.
Letang was placed on injured reserve on Monday but activated in time for Tuesday's game against the Montreal Canadiens, prompting the demotion of defenceman Simon Depres.
With Letang being placed back on injured reserve, Depres has been called back up.
The Penguins blanked the Canadiens, 1-0 to extend their win streak to 13 games.